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25-Oct-2020 08:05

These observations raise the possibility of improving the treatment of Hirschsprung disease by transplanting NCSCs into the aganglionic portion of the gut to generate enteric ganglia by bypassing the migrating defects.

Flow-cytometrically purified NCSCs from the ENS (gut NCSCs) migrated into the developing gut and formed enteric neurons.

During development, enteric neurons are generated asynchronously.

Serotonergic neurons, which arise early, affect development/survival of later-born dopaminergic, gamma-aminobutyric acidergic, nitrergic, and calcitonin gene-related peptide-expressing neurons and are essential for gastrointestinal motility.

However, neuroepithelial stem cells (NESCs) harvested from the neural tube of embryonic rats can differentiate into NCSCs, which in turn generate peripheral nervous system derivatives.

Addition of 5-HT to cultures of isolated enteric neural crest-derived cells (ENCDCs) promoted total and dopaminergic neuronal development.

NC cells are stem cells that are specified within the embryonic neuroectodermal epithelium and migrate to stereotyped peripheral sites for differentiation into many cell types.



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